利用大肠杆菌单链结合蛋白在Taq DNA聚合酶PCR过程中减少缺失诱变假象 - Suppr

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超能文献

Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB.

作者信息

Chou Q

机构信息

Cetus Corporation, Emeryville, CA 94608.

出版信息

Nucleic Acids Res. 1992 Aug 25;20(16):4371. doi: 10.1093/nar/20.16.4371.

DOI:10.1093/nar/20.16.4371

PMID:1508731

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334154/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd39/334154/8ed75395aee4/nar00227-0246-a.jpg

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Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB.利用大肠杆菌单链结合蛋白在Taq DNA聚合酶PCR过程中减少缺失诱变假象

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本文引用的文献

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.使用改良的噬菌体T7 DNA聚合酶进行DNA序列分析。

Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71. doi: 10.1073/pnas.84.14.4767.

Deletion mutagenesis during polymerase chain reaction: dependence on DNA polymerase.聚合酶链式反应期间的缺失诱变：对DNA聚合酶的依赖性。

Gene. 1991 Mar 1;99(1):105-8. doi: 10.1016/0378-1119(91)90040-i.

Sequence of the cDNA encoding ovine tumor necrosis factor-alpha: problems with cloning by inverse PCR.编码绵羊肿瘤坏死因子-α的cDNA序列：反向PCR克隆的问题

Gene. 1991 Dec 30;109(2):203-10. doi: 10.1016/0378-1119(91)90610-n.

Use of an RNA folding algorithm to choose regions for amplification by the polymerase chain reaction.

Anal Biochem. 1990 Feb 15;185(1):57-62. doi: 10.1016/0003-2697(90)90254-7.

Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.预防PCR前的错配引物和引物二聚体形成可改善低拷贝数扩增。

Nucleic Acids Res. 1992 Apr 11;20(7):1717-23. doi: 10.1093/nar/20.7.1717.

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