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Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB.

作者信息

Chou Q

机构信息

Cetus Corporation, Emeryville, CA 94608.

出版信息

Nucleic Acids Res. 1992 Aug 25;20(16):4371. doi: 10.1093/nar/20.16.4371.

DOI:10.1093/nar/20.16.4371
PMID:1508731
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334154/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd39/334154/8ed75395aee4/nar00227-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd39/334154/8ed75395aee4/nar00227-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd39/334154/8ed75395aee4/nar00227-0246-a.jpg

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本文引用的文献

1
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.使用改良的噬菌体T7 DNA聚合酶进行DNA序列分析。
Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71. doi: 10.1073/pnas.84.14.4767.
2
Deletion mutagenesis during polymerase chain reaction: dependence on DNA polymerase.聚合酶链式反应期间的缺失诱变：对DNA聚合酶的依赖性。
Gene. 1991 Mar 1;99(1):105-8. doi: 10.1016/0378-1119(91)90040-i.
3
Sequence of the cDNA encoding ovine tumor necrosis factor-alpha: problems with cloning by inverse PCR.编码绵羊肿瘤坏死因子-α的cDNA序列：反向PCR克隆的问题
单分子 DREEM 成像揭示了 DNA 缠绕在人线粒体单链 DNA 结合蛋白周围。
Nucleic Acids Res. 2018 Nov 30;46(21):11287-11302. doi: 10.1093/nar/gky875.
4
Development of nanoparticle-assisted PCR assay in the rapid detection of brain-eating amoebae.用于快速检测食脑变形虫的纳米颗粒辅助PCR检测方法的开发。
Parasitol Res. 2018 Jun;117(6):1801-1811. doi: 10.1007/s00436-018-5864-0. Epub 2018 Apr 20.
5
Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots.通过碲化镉量子点增强多重聚合酶链反应的特异性
Nanoscale Res Lett. 2011 Dec;6(1):51. doi: 10.1007/s11671-010-9797-5. Epub 2010 Sep 30.
6
DMSO and betaine greatly improve amplification of GC-rich constructs in de novo synthesis.DMSO 和甜菜碱极大地提高了从头合成中富含 GC 结构的扩增。
PLoS One. 2010 Jun 11;5(6):e11024. doi: 10.1371/journal.pone.0011024.
7
Enhanced protocol for determining the 3' terminus of hepatitis C virus.增强型丙型肝炎病毒 3'末端测定法。
J Virol Methods. 2010 Aug;167(2):158-64. doi: 10.1016/j.jviromet.2010.03.030. Epub 2010 Apr 8.
8
Effect of PCR amplicon size on assessments of clone library microbial diversity and community structure.聚合酶链式反应（PCR）扩增子大小对克隆文库微生物多样性和群落结构评估的影响。
Environ Microbiol. 2009 May;11(5):1292-302. doi: 10.1111/j.1462-2920.2008.01857.x. Epub 2009 Feb 9.
9
Thermus thermophilus as biological model.嗜热栖热菌作为生物学模型。
Extremophiles. 2009 Mar;13(2):213-31. doi: 10.1007/s00792-009-0226-6. Epub 2009 Jan 21.
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