Zhang Dong, Li Yingcun, Zhang Xuange, Cheng Yongqiang, Li Zhengping
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University Baoding 071002 Hebei P. R. China
Sports Science Institute of Hebei Province Shijiazhuang 050011 Hebei P. R. China.
RSC Adv. 2019 Mar 26;9(17):9373-9378. doi: 10.1039/c8ra09689a. eCollection 2019 Mar 22.
In this paper, we demonstrated that the polymerase chain reaction (PCR) could be dramatically enhanced by tungsten disulfide (WS). The results showed that the PCR efficiency could be increased with the addition of WS and at a lower annealing temperature, which simplified the design and operation of PCR. Moreover, PCR with WS showed better specificity and efficiency as compared with graphene oxide (GO) for a human genome DNA sample. The mechanism of enhancement of PCR by WS was discussed according to the typical structure and the characteristics of selective adsorption of single-stranded DNA by WS. The results suggested that WS as a PCR enhancer can promote the PCR performance and extend the PCR application in biomedical research, clinical diagnostic, and bioanalysis.
在本文中，我们证明了二硫化钨（WS）可显著增强聚合酶链反应（PCR）。结果表明，添加WS并在较低退火温度下，PCR效率可提高，这简化了PCR的设计与操作。此外，对于人类基因组DNA样本，与氧化石墨烯（GO）相比，含WS的PCR表现出更好的特异性和效率。根据WS的典型结构以及其对单链DNA的选择性吸附特性，探讨了WS增强PCR的机制。结果表明，WS作为一种PCR增强剂可提升PCR性能，并拓展其在生物医学研究、临床诊断和生物分析中的应用。
