Gururaj H B, Giridhar P, Ravishankar G A
Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore 570 020, India.
Indian J Exp Biol. 2004 Apr;42(4):424-8.
A highly efficient two stage protocol was developed for induction of multiple shoots from single node in vitro shoot tip explants of Decalepis hamiltonii. It was found that phloroglucinol (PG) had synergistic effect on shoot multiplication when added with N6-benzyladenine and gibberellic acid. This protocol uses PG for both multiple shoot induction from nodal explants, elongation of primary shoots and initiation of adventitious shoot formation from primary shoots, which was more in presence of triacontanol (TRIA). Maximum number of shoots per culture was observed on the medium containing N6-benzyladenine (1.1 microM; BA), GA3 (5.8 microM) and PG (800 microM). Sub-culturing of the shoots onto MS medium containing optimum concentration of BA (5.6 microM), PG (200 microM) and TRIA (0.011 microM) produced elongated shoots along with secondary shoot formation. The long shoots were rooted on alpha-naphthalene acetic acid (5.38 microM; NAA) and PG (400 microM) containing medium. The rooted plantlets were hardened and their field survival rate was 80-90%.
为从汉氏十萼茄离体茎尖外植体的单节段诱导丛生芽，开发了一种高效的两阶段方案。研究发现，间苯三酚（PG）与N6-苄基腺嘌呤和赤霉素一起添加时，对芽增殖具有协同作用。该方案使用PG进行节段外植体的丛生芽诱导、初生芽伸长以及初生芽不定芽形成的起始，在含有三十烷醇（TRIA）的情况下效果更佳。在含有N6-苄基腺嘌呤（1.1微摩尔；BA）、GA3（5.8微摩尔）和PG（800微摩尔）的培养基上观察到每个培养物中芽的最大数量。将芽继代培养到含有最佳浓度BA（5.6微摩尔）、PG（200微摩尔）和TRIA（0.011微摩尔）的MS培养基上，可产生伸长的芽并伴有次生芽形成。长芽在含有α-萘乙酸（5.38微摩尔；NAA）和PG（400微摩尔）的培养基上生根。生根的小植株经过炼苗，其田间成活率为80-90%。
