Tan Si Nan, Tee Chong Siang, Wong Hann Ling
Department of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia.
Plant Biotechnol (Tokyo). 2018 Dec 25;35(4):325-334. doi: 10.5511/plantbiotechnology.18.0711a.
is a legume plant which has great potential to be used as a biofuel feedstock. Conventional propagation of was found to be inefficient for mass propagation. Employing plant tissue culture techniques for micropropagation and further plant improvement of will be the right path to fulfill future challenges in biofuel production. This study aimed to establish a plant regeneration system for potential micropropagation and genetic manipulation of in future. In vitro nodal explants were used and Woody Plant Medium (WPM) containing 30 µM 6-benzylaminopurine (BAP) and 1 mM phloroglucinol (PG) was able to induce higher frequency of multiple shoot buds compared to other media investigated in this study. For shoot regeneration study, WPM containing 15 µM of zeatin and 1 mM PG was able to induce longer shoots while rooting of the regenerated shoots was enhanced by WPM supplemented with indole-3-butyric acid (IBA) in combination with silver thiosulphate (STS). A simple and effective acclimatisation protocol was established with very high survival frequency of regenerated plantlets. Root nodulation of the successfully acclimatised plants was also observed. In short, multiple shoot buds were successfully induced, regenerated and rooted in vitro. The rooted plantlets were successfully acclimatised and grown healthily. It was concluded that a successful plant regeneration protocol of was achieved for potential application in micropropagation and genetic manipulation.
是一种具有巨大潜力可作为生物燃料原料的豆科植物。已发现传统的繁殖方式在大规模繁殖方面效率低下。采用植物组织培养技术进行微繁殖并进一步改良该植物，将是应对未来生物燃料生产挑战的正确途径。本研究旨在建立一个用于未来对该植物进行潜在微繁殖和基因操作的植物再生系统。使用了离体茎节外植体，与本研究中调查的其他培养基相比，含有30 μM 6-苄基腺嘌呤（BAP）和1 mM间苯三酚（PG）的木本植物培养基（WPM）能够诱导更高频率的多芽。对于芽再生研究，含有15 μM玉米素和1 mM PG的WPM能够诱导出更长的芽，而再生芽的生根则通过添加吲哚-3-丁酸（IBA）与硫代硫酸银（STS）组合的WPM得到增强。建立了一个简单有效的驯化方案，再生植株的成活率非常高。还观察到成功驯化的植株有根瘤形成。简而言之，多芽在体外成功诱导、再生并生根。生根的植株成功驯化并健康生长。得出的结论是，已实现了该植物成功的植物再生方案，可用于潜在的微繁殖和基因操作。
