用于分析乳制品中有机酸的反相高效液相色谱替代方法。
Alternative reversed-phase high-performance liquid chromatography method to analyse organic acids in dairy products.
作者信息
Tormo M, Izco J M
机构信息
Instituto Lactológico de Lekunberri, Plazaola 23, Lekunberri, Navarra 31870, Spain.
出版信息
J Chromatogr A. 2004 Apr 16;1033(2):305-10. doi: 10.1016/j.chroma.2004.01.043.
A RP-HPLC method for the analysis of oxalic, citric, formic, succinic, orotic, uric, pyruvic, acetic, propionic, lactic and butyric acids in dairy products with a simple treatment of the sample has been developed. A gradient programme pumping phosphate buffer at pH 2.20 and acetonitrile was used to separate the compounds on a C18 column. Various parameters affecting analysis have been optimised to take < 18 min with an excellent linearity (R > 0.999). The precision was good (R.S.D. < 5%) and the recovery found close to 100%. Its application to analyse the quality of some dairy products has been investigated.
已开发出一种反相高效液相色谱（RP-HPLC）方法，用于分析乳制品中的草酸、柠檬酸、甲酸、琥珀酸、乳清酸、尿酸、丙酮酸、乙酸、丙酸、乳酸和丁酸。该方法对样品处理简单，采用梯度洗脱程序，以pH 2.20的磷酸盐缓冲液和乙腈为流动相，在C18柱上分离这些化合物。对影响分析的各种参数进行了优化，分析时间小于18分钟，线性良好（R>0.999）。精密度良好（相对标准偏差<5%），回收率接近100%。已对该方法在分析某些乳制品质量方面的应用进行了研究。
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