Robson W C, Katz R W
University of Michigan School of Dentistry, Ann Arbor.
Proc Finn Dent Soc. 1992;88 Suppl 1:279-83.
The use of osteoinductive demineralized dentin matrix in the repair of vital pulp exposures was examined. The pulps of the maxillary first molars of adult male Long Evans rats were exposed and capped with either demineralized dentin particles or guanidinium extracted demineralized dentin particles; then covered with a glass ionomer cement restoration. The animals were sacrificed after two weeks and the maxillae were dissected free, fixed in formaldehyde and demineralized. Histologic examination revealed that in those cases where the protective glass ionomer restoration was intact, both groups showed some healing. There was some preliminary indication that the extent of reactive matrix formation was greater in the osteoinductive group. In both groups, odontoblast-like cells appeared to incorporate some of the dentin particles into the reparative dentin bridge. This preliminary work suggests that osteoinductive factors present in demineralized incisor dentin might be used to guide the formation of a dentin bridge in a rat model for vital pulp exposures.
研究了骨诱导性脱矿牙本质基质在活髓暴露修复中的应用。将成年雄性Long Evans大鼠上颌第一磨牙的牙髓暴露，并用脱矿牙本质颗粒或胍提取的脱矿牙本质颗粒覆盖；然后用玻璃离子水门汀修复体覆盖。两周后处死动物，分离出上颌骨，固定于甲醛中并脱矿。组织学检查显示，在那些保护性玻璃离子修复体完整的病例中，两组均有一定程度的愈合。有初步迹象表明，骨诱导组中反应性基质形成的程度更大。在两组中，成牙本质细胞样细胞似乎将一些牙本质颗粒纳入修复性牙本质桥中。这项初步研究表明，脱矿切牙本质中存在的骨诱导因子可能用于在大鼠活髓暴露模型中引导牙本质桥的形成。
