培养的血管内皮细胞中细胞内钙升高与前列环素（PGI2）生成之间的相关性。

The correlation between rises in intracellular calcium and PGI2 production in cultured vascular endothelial cells.

作者信息

Watanabe K, Lam G, Jaffe E A

机构信息

Department of Medicine, Cornell University Medical College, New York, NY 10021.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 1992 Jul;46(3):211-4. doi: 10.1016/0952-3278(92)90072-q.

DOI:10.1016/0952-3278(92)90072-q

PMID:1508953

Abstract

Elevation of intracellular calcium in response to trypsin, bradykinin, thrombin or histamine is associated with a proportional increase in PGI2 production in cultured human umbilical vein endothelial cells (HUVEC), bovine pulmonary artery endothelial cells (CPAE), and bovine aortic endothelial cells (BAEC). The major agonists that induce increases in intracellular calcium and PGI2 production are thrombin and trypsin in HUVEC, bradykinin in CPAE, and bradykinin and trypsin in BAEC. These results suggest that endothelial cells derived from different species or sites require different agonists to induce increases in intracellular calcium and PGI2 production and that only agonists which increase intracellular calcium can stimulate PGI2 production.

摘要

在培养的人脐静脉内皮细胞（HUVEC）、牛肺动脉内皮细胞（CPAE）和牛主动脉内皮细胞（BAEC）中，对胰蛋白酶、缓激肽、凝血酶或组胺作出反应时细胞内钙的升高与前列环素（PGI2）生成的相应增加相关。在HUVEC中，诱导细胞内钙和PGI2生成增加的主要激动剂是凝血酶和胰蛋白酶；在CPAE中是缓激肽；在BAEC中是缓激肽和胰蛋白酶。这些结果表明，源自不同物种或部位的内皮细胞需要不同的激动剂来诱导细胞内钙和PGI2生成增加，并且只有能增加细胞内钙的激动剂才能刺激PGI2的生成。