Van Assche F, Clijsters H
Department SBM, Limburgs Universitair Centrum, Universitaire Campus, B-3610 Diepenbeek, Belgium.
Environ Pollut. 1990;66(2):157-72. doi: 10.1016/0269-7491(90)90118-v.
The potential soil phytotoxicity in the surroundings of an old zinc smelter closed in 1966, was evaluated by a biological test system. This system is based on the analysis of shoot growth and (iso-) enzymes in leaves and roots of 2-week-old seedlings of Phaseolus vulgaris grown on soil samples under controlled environmental conditions. The biological data were presented in a phytotoxicity index, which allowed classification of the soil samples into four phytotoxicity classes. Comparison was made between the biological results and soil and plant metal content. The study revealed that more than 20 years after closure of the factory, the soils in its surroundings were still potentially phytotoxic. Zinc proved to be the most important, but not the only, phytotoxic element, as was shown by isozyme analysis and by the strong correlation between the biological data, the water soluble Zn fraction of the soil and leaf Zn content. The effect of contamination extended to a distance of more than 3 km from the industrial site in the direction of the prevailing wind.
通过生物测试系统对1966年关闭的一座旧锌冶炼厂周边土壤的潜在植物毒性进行了评估。该系统基于对在可控环境条件下生长于土壤样品上的2周龄菜豆幼苗的地上部生长以及叶片和根系中的（同工）酶进行分析。生物数据以植物毒性指数呈现，该指数可将土壤样品分为四个植物毒性等级。对生物结果与土壤及植物金属含量进行了比较。研究表明，工厂关闭20多年后，其周边土壤仍具有潜在的植物毒性。同工酶分析以及生物数据、土壤水溶性锌组分和叶片锌含量之间的强相关性表明，锌被证明是最重要但并非唯一的植物毒性元素。污染影响范围在盛行风方向上延伸至距工业场地3公里以外。
