Kiyosawa Naoki, Ito Kazumi, Niino Noriyo, Sakuma Kyoko, Kanbori Miyuki, Yamoto Takashi, Manabe Sunao, Matsunuma Naochika
Medicinal Safety Research Labs., Sankyo Co. Ltd., 717 Horikoshi, Fukuroi, Shizuoka 437-0065, Japan.
Toxicol Lett. 2004 Apr 21;150(2):157-66. doi: 10.1016/j.toxlet.2004.01.004.
Genes that showed mRNA content profiles, which correlated with serum concentrations of total cholesterol (T.CHO), were screened from the microarray data of phenobarbital (PB)- or clofibrate (CLO)-treated rat livers, and the correlation was evaluated based on Spearman's correlation coefficient. Many genes involved in the cholesterol or bile acid metabolism were highly correlated such as UDP-glucuronosyltransferase-21, apolipoprotein A-I and cMOAT. The mRNA content of the amyloid precursor protein (APP) showed the 5th highest correlation among the 8799 probes in the Affymetrix Rat Genome U34 Array. In the livers of rats fed a high-cholesterol (1%) diet for 33 days, serum T.CHO levels increased by 4.6-fold, and the hepatic APP mRNA content also increased by 1.9-fold compared to the control group. These data suggest that the hepatic APP mRNA content was affected by serum T.CHO, and that hepatic APP was involved in cholesterol metabolism in rat livers.
从苯巴比妥（PB）或氯贝丁酯（CLO）处理的大鼠肝脏微阵列数据中筛选出mRNA含量谱与血清总胆固醇（T.CHO）浓度相关的基因，并基于斯皮尔曼相关系数评估相关性。许多参与胆固醇或胆汁酸代谢的基因高度相关，如尿苷二磷酸葡萄糖醛酸基转移酶-21、载脂蛋白A-I和cMOAT。淀粉样前体蛋白（APP）的mRNA含量在Affymetrix大鼠基因组U34阵列的8799个探针中显示出第五高的相关性。在喂食高胆固醇（1%）饮食33天的大鼠肝脏中，血清T.CHO水平增加了4.6倍，与对照组相比，肝脏APP mRNA含量也增加了1.9倍。这些数据表明肝脏APP mRNA含量受血清T.CHO影响，且肝脏APP参与大鼠肝脏的胆固醇代谢。
