Bowen Suzanne, Wheals Alan E
Department of Genetics, University of Leicester, Leicester LE1 7RH, UK.
FEMS Yeast Res. 2004 May;4(7):731-5. doi: 10.1016/j.femsyr.2004.02.006.
KRE6 (YPR159W) encodes a Golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. A functional Kre6p is necessary for cell wall protein accumulation in response to changing metabolic conditions. The product of the SED1 (YDR077W) gene is a stress-induced GPI-cell wall protein. Successful incorporation of HA-tagged Sed1p into the cell wall involves KRE6. The double-mutant sed1 kre6 has a reduced growth rate, increased flocculation and increased sensitivity to Zymolyase. A similar phenotype is found in mutants defective in glycosyl-phosphatidyl-insositol (GPI) anchor assembly. These findings support the theory that Kre6p could function as a transglucosylase that allows the incorporation of proteins with a GPI anchor into the cell wall.
KRE6（YPR159W）编码一种高尔基体膜蛋白，该蛋白是细胞壁中正常β-1,6-葡聚糖水平所必需的。功能性Kre6p对于响应代谢条件变化的细胞壁蛋白积累是必要的。SED1（YDR077W）基因的产物是一种应激诱导的糖基磷脂酰肌醇（GPI）-细胞壁蛋白。将HA标记的Sed1p成功整合到细胞壁中需要KRE6。双突变体sed1 kre6的生长速率降低、絮凝增加且对溶壁酶的敏感性增加。在糖基磷脂酰肌醇（GPI）锚定组装缺陷的突变体中也发现了类似的表型。这些发现支持了Kre6p可以作为一种转葡糖基酶发挥作用的理论，该酶允许将具有GPI锚定的蛋白质整合到细胞壁中。
