Terletski Valeri, Michael Geovana Brenner, Schwarz Stefan
Institute for Animal Breeding, Federal Agricultural Research Center (FAL), Höltystrasse 10, 31535 Neustadt-Mariensee, Germany.
FEMS Immunol Med Microbiol. 2004 May 1;41(1):1-8. doi: 10.1016/j.femsim.2004.01.009.
Molecular typing of bacterial pathogens is an important issue in the epidemiological analysis of emerging infections in humans and animals. Numerous methods have been developed for and applied to a wide variety of bacteria of medical, veterinary and zoonotic importance. The present minireview provides a description of a new typing approach designated subtracted restriction fingerprinting (SRF), its use for typing of Salmonella isolates and a comparison with the most widely used typing techniques for these bacteria. SRF is based on double restriction endonuclease digestion of whole cell DNA, followed by a fill-in reaction with specifically tagged nucleotides and subtractive capture of selected restriction fragments. This results in a reduced number of fragments optimal for separation in standard agarose gels.
