Lindholm T, Sköld M K, Suneson A, Carlstedt T, Cullheim S, Risling M
Swedish Defence Research Agency, Experimental Traumatology, Stockholm, Sweden.
Neuroreport. 2004 Mar 22;15(4):649-54. doi: 10.1097/00001756-200403220-00015.
We have examined mRNA and protein distribution for the axon guidance molecules semaphorin3A, 3F, 4F and semaphorin receptors neuropilin-1 and 2, 1-21 days after intramedullary axotomy of rat lumbar spinal cord motoneurons. We show that semaphorin3A mRNA and protein are up-regulated in the scar and in motoneurons from 3 days and upto 3 weeks after injury. Neuropilin-1 mRNA showed no changed expression in axotomized motoneurons. Semaphorin3F mRNA expression was found in ventral roots after ventral funiculus lesion (VFL) and neuropilin-2 mRNA was found in affected motoneurons from 1 day after injury throughout the examined period. Semaphorin4F mRNA was first found in motoneurons 3 weeks after lesion. These results suggest semaphorin/neuropilin involvement in the injury response of intramedullary axotomized motoneurons.
我们检测了大鼠腰段脊髓运动神经元髓内轴突切断术后1 - 21天，轴突导向分子信号素3A、3F、4F以及信号素受体神经纤毛蛋白-1和2的mRNA和蛋白分布。我们发现，损伤后3天至3周，信号素3A的mRNA和蛋白在瘢痕组织和运动神经元中上调。神经纤毛蛋白-1的mRNA在轴突切断的运动神经元中表达无变化。在腹侧索损伤（VFL）后的腹根中发现了信号素3F的mRNA表达，在损伤后1天直至整个检测期间，在受影响的运动神经元中发现了神经纤毛蛋白-2的mRNA。损伤后3周首次在运动神经元中发现信号素4F的mRNA。这些结果表明信号素/神经纤毛蛋白参与了髓内轴突切断的运动神经元的损伤反应。
