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大鼠脾脏滤泡中的树突状细胞。一项免疫组织化学与酶组织化学相结合的研究。

Dendritic cells in the rat spleen follicles. A combined immuno- and enzyme histochemical study.

作者信息

Eikelenboom P

出版信息

Cell Tissue Res. 1978 Jun 26;190(1):79-87. doi: 10.1007/BF00210038.

DOI:10.1007/BF00210038
PMID:150949
Abstract

Follicles of peripheral lymphoid organs (rat) contain a type of non-lymphoid cell which is capable of arresting antigen-antibody complexes at the cell surface. These so-called dendritic cells can be visualized in immunized rats by staining antigen-antibody complexes with immunohistoperoxidase techniques. The present study concerns a classification of these cells and comparison with known non-lymphoid cell types such as macrophages, marginal metallophils and tingible body macrophages in the rat spleen follicles. Immunoenzyme histochemical and (enzyme) histochemical techniques have been combined in the same tissue sections to correlate the functional capacity of binding immune complexes with morphological characteristics or phagocytic capacity. Dendritic cells show silver affinity but do not demonstrate a characteristic pattern of hydrolytic enzymes or phagocytosis.

摘要

外周淋巴器官(大鼠)的滤泡含有一种非淋巴细胞,这种细胞能够在细胞表面捕获抗原-抗体复合物。通过免疫组化过氧化物酶技术对抗原-抗体复合物进行染色,可在免疫大鼠中观察到这些所谓的树突状细胞。本研究涉及这些细胞的分类,并与大鼠脾滤泡中已知的非淋巴细胞类型(如巨噬细胞、边缘嗜金属细胞和可染小体巨噬细胞)进行比较。免疫酶组织化学和(酶)组织化学技术已结合在同一组织切片中,以将结合免疫复合物的功能能力与形态特征或吞噬能力相关联。树突状细胞显示出银亲和力,但未表现出水解酶或吞噬作用的特征模式。

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引用本文的文献

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A COMPARISON OF THE METALOPHILIC RETICULOENDOTHELIAL CELLS TO CELLS CONTAINING ACID PHOSPHATASE AND NON-SPECIFIC ESTERASE IN THE LYMPHOID NODULES OF NORMAL AND STIMULATED RAT SPLEENS.正常及受刺激大鼠脾脏淋巴小结中嗜金属性网状内皮细胞与含酸性磷酸酶和非特异性酯酶细胞的比较
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