Corsaro M M, Piaz F Dal, Lanzetta R, Naldi T, Parrilli M
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Complesso Universitario Monte S Angelo, Via Cynthia 4, 80126 Napoli, Italy.
Rapid Commun Mass Spectrom. 2004;18(8):853-8. doi: 10.1002/rcm.1414.
The use of the electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-QTOFMS) technique for the structural determination of Lipid A from Pseudomonas corrugata is described. This technique appears to be more sensitive with respect to other commonly used tandem mass spectrometric approaches, and was very valuable in the structural determination of the highly heterogeneous Lipid A fractions. The Lipid A fraction consists mainly of a pentaacyl component in which 3-hydroxydecanoyl [10:0(3-OH)] and 3-hydroxydodecanoyl [12:0(3-OH)] are linked as primary acyl substituents to the classical bisphosphorylated beta-(1' --> 6)-linked D-glucosamine disaccharide. Secondary substitution of N-acyl fatty acids with dodecanoyl residues [12:0] and/or its 2-OH derivatives was also observed.
描述了使用电喷雾电离四极杆飞行时间串联质谱（ESI-QTOFMS）技术来确定皱折假单胞菌脂质A的结构。相对于其他常用的串联质谱方法，该技术似乎更灵敏，并且在高度异质的脂质A组分的结构测定中非常有价值。脂质A组分主要由五酰基成分组成，其中3-羟基癸酰基[10:0(3-OH)]和3-羟基十二酰基[12:0(3-OH)]作为主要酰基取代基连接到经典的双磷酸化β-(1'→6)-连接的D-葡萄糖胺二糖上。还观察到N-酰基脂肪酸被十二酰基残基[12:0]和/或其2-OH衍生物进行二级取代。
