Paganoni Sabrina, Anderson Kelsi L, Ferreira Adriana
Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
Glia. 2004 May;46(4):456-66. doi: 10.1002/glia.20023.
Ror1 and Ror2 belong to a family of tyrosine kinase receptors that are highly conserved among species. They are expressed throughout the organism, including the central nervous system. In the present study, we analyzed the expression and subcellular localization of Ror1 and Ror2 in astrocytes by means of reverse transcription-polymerase chain reaction, Western blot analysis, and immunocytochemistry. Our results indicated that both Ror1 and Ror2 are readily detectable in cultured astrocytes. They also showed that Ror1 and Ror2 are associated with different components of the cytoskeleton. While Ror1 co-localized with F-actin along stress fibers, Ror2 partially co-localized with microtubules. In addition, our results suggest that Ror1 and Ror2 undergo different posttranslational modifications in cultured astrocytes. Ror1 is highly glycosylated in these cells. In contrast, no glycosylation was detected in Ror2. Taken together, these results suggest distinct roles for these tyrosine kinase receptors in astrocytes.
Ror1和Ror2属于一类酪氨酸激酶受体家族，在物种间高度保守。它们在整个机体中表达，包括中枢神经系统。在本研究中，我们通过逆转录-聚合酶链反应、蛋白质免疫印迹分析和免疫细胞化学方法，分析了Ror1和Ror2在星形胶质细胞中的表达及亚细胞定位。我们的结果表明，在培养的星形胶质细胞中，Ror1和Ror2均易于检测到。结果还显示，Ror1和Ror2与细胞骨架的不同成分相关。Ror1沿着应力纤维与F-肌动蛋白共定位，而Ror2则部分与微管共定位。此外，我们的结果表明，Ror1和Ror2在培养的星形胶质细胞中经历不同的翻译后修饰。Ror1在这些细胞中高度糖基化。相比之下，未在Ror2中检测到糖基化。综上所述，这些结果表明这些酪氨酸激酶受体在星形胶质细胞中具有不同的作用。
