Paganoni Sabrina, Ferreira Adriana
Institute for Neuroscience, Northwestern University, Chicago, Illinois 60611, USA.
J Neurosci Res. 2003 Aug 15;73(4):429-40. doi: 10.1002/jnr.10674.
Ror1 and Ror2 are two novel receptor tyrosine kinases that have been implicated in neuronal differentiation in Caenorhabditis elegans. As a first step toward elucidating their role in the mammalian brain, we analyzed their expression and localization patterns in hippocampal neurons. Our results showed that both receptors are expressed from early stages of development and that their protein levels peak during periods of active synapse formation. Immunocytochemical analysis indicated that Ror1 and Ror2 are highly concentrated in the growth cones of immature neurons and are present throughout the somatodendritic compartment of mature hippocampal cells. Further analysis indicated that they are present not only in the cell membrane but also in Triton- and saponin-insoluble fractions, suggesting that they may be associated with both the cytoskeleton and membrane-bound organelles. Taken collectively, our results suggest that Ror1 and Ror2 might play a role during early stages of development in mammalian central neurons.
Ror1和Ror2是两种新型受体酪氨酸激酶，它们与秀丽隐杆线虫的神经元分化有关。作为阐明它们在哺乳动物大脑中作用的第一步，我们分析了它们在海马神经元中的表达和定位模式。我们的结果表明，这两种受体在发育早期就开始表达，并且它们的蛋白质水平在活跃的突触形成期达到峰值。免疫细胞化学分析表明，Ror1和Ror2高度集中在未成熟神经元的生长锥中，并且存在于成熟海马细胞的整个树突状细胞区室中。进一步分析表明，它们不仅存在于细胞膜中，还存在于不溶于 Triton 和皂素的组分中，这表明它们可能与细胞骨架和膜结合细胞器都有关联。综合来看，我们的结果表明Ror1和Ror2可能在哺乳动物中枢神经元发育的早期阶段发挥作用。
