Smith Richard D, Shen Yufeng, Tang Keqi
Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA.
Acc Chem Res. 2004 Apr;37(4):269-78. doi: 10.1021/ar0301330.
This article describes developments in fundamental and applied aspects of separations, electrospray ionization phenomena, and mass spectrometric instrumentation that are interrelated and important for making more effective and quantitative measurements, particularly for proteomics applications. The basis for better quantitation and ultrahigh sensitivity is highlighted for high-resolution capillary liquid chromatography separations that provide low nanoliter per minute flow rates to an electrospray ionization interface. The increased dynamic range of measurements and low zeptomole regime detection limits obtainable open new avenues for biological research.
本文描述了分离、电喷雾电离现象以及质谱仪器在基础和应用方面的发展，这些发展相互关联且对于进行更有效和定量的测量非常重要，特别是在蛋白质组学应用中。重点强调了高分辨率毛细管液相色谱分离为电喷雾电离接口提供每分钟低纳升流速时，实现更好定量和超高灵敏度的基础。测量动态范围的增加以及可达到的低zeptomole水平检测限为生物学研究开辟了新途径。
