Wilson Rashaun S, Nairn Angus C
Yale/NIDA Neuroproteomics Center, 300 George St., New Haven, CT 06511, USA.
Department of Psychiatry, Yale School of Medicine, Connecticut Mental Health Center, New Haven, CT 06511, USA.
Proteomes. 2018 Dec 9;6(4):51. doi: 10.3390/proteomes6040051.
Cell-type-specific analysis has become a major focus for many investigators in the field of neuroscience, particularly because of the large number of different cell populations found in brain tissue that play roles in a variety of developmental and behavioral disorders. However, isolation of these specific cell types can be challenging due to their nonuniformity and complex projections to different brain regions. Moreover, many analytical techniques used for protein detection and quantitation remain insensitive to the low amounts of protein extracted from specific cell populations. Despite these challenges, methods to improve proteomic yield and increase resolution continue to develop at a rapid rate. In this review, we highlight the importance of cell-type-specific proteomics in neuroscience and the technical difficulties associated. Furthermore, current progress and technological advancements in cell-type-specific proteomics research are discussed with an emphasis in neuroscience.
细胞类型特异性分析已成为神经科学领域许多研究人员的主要关注点，特别是因为在脑组织中发现了大量不同的细胞群体，它们在各种发育和行为障碍中发挥作用。然而，由于这些特定细胞类型的不均匀性以及它们向不同脑区的复杂投射，分离它们可能具有挑战性。此外，许多用于蛋白质检测和定量的分析技术对从特定细胞群体中提取的少量蛋白质仍然不敏感。尽管存在这些挑战，但提高蛋白质组学产量和增加分辨率的方法仍在快速发展。在这篇综述中，我们强调了细胞类型特异性蛋白质组学在神经科学中的重要性以及相关的技术难题。此外，还讨论了细胞类型特异性蛋白质组学研究的当前进展和技术进步，重点是神经科学。
