Woycechowsky Kenneth J, Hilvert Donald
Laboratorium für Organische Chemie, Swiss Federal Institute of Technology, ETH-Hönggerberg, Zürich, Switzerland.
Eur J Biochem. 2004 May;271(9):1630-7. doi: 10.1111/j.1432-1033.2004.04073.x.
The efficient engineering of enzymes with novel activities remains an ongoing challenge. Towards this end, genetic selection techniques provide a method for finding rare solutions to catalytic problems that requires only a limited foreknowledge of structure-function relationships. We have used genetic selections to extensively probe the structure and mechanism of chorismate mutases. The insights gained from these investigations will aid future enzyme design efforts.
高效构建具有新活性的酶仍然是一个持续存在的挑战。为此，基因筛选技术提供了一种方法，用于找到催化问题的罕见解决方案，而这仅需要对结构-功能关系有有限的先验知识。我们已利用基因筛选广泛探究了分支酸变位酶的结构和机制。从这些研究中获得的见解将有助于未来的酶设计工作。
