Ohlsson Marcus, Mattsson Per, Svensson Mikael
Department of Clinical Neuroscience, Section of Neurosurgery, Karolinska Institutet and Hospital, Stockholm, Sweden.
Restor Neurol Neurosci. 2004;22(1):1-10.
In this methodological study, we describe a standardized optic nerve injury model in adult rats. The temporal pattern of the glial response is mapped in relation to axonal regeneration and degeneration.
A standardized optic nerve crush injury was made in adult rats, using a fine pair of forceps with a preset pressure of 0.6 N during 10 seconds.
This crush injury resulted in a pronounced astroglial and microglial proliferation 2-7 dpi. Degenerative axons distal to the lesion presented a gradual decrease in neurofilament immunoreactivity. In contrast, Fluoro-Jade staining was gradually increased in these degenerative axons from 2-7 dpi. Regeneration was observed proximal to the lesion in sprouts double labeled with the tracer Fluoro-Gold and anti-GAP43.
The standardized optic nerve crush injury model used in the present study resulted in a reproducible degenerative pattern including a pronounced astroglial and microglial proliferation. The gradual decrease in Neurofilament immunoreactivity in degenerative axons, and a gradual increase in Fluoro-Jade staining was paralleled with a spontaneous regeneration proximal to the lesion, shown with tracing and GAP43-immunoreactivity. The combination of these markers and this experimental setup thus allows analysis of the entire degenerative/regenerative process in a standardized CNS axotomy model.
在本方法学研究中，我们描述了一种成年大鼠标准化视神经损伤模型。绘制了胶质细胞反应的时间模式与轴突再生和变性的关系。
使用一对精细镊子，在成年大鼠中造成标准化视神经挤压伤，在10秒内预设压力为0.6N。
这种挤压伤在损伤后2 - 7天导致明显的星形胶质细胞和小胶质细胞增殖。损伤远端的变性轴突神经丝免疫反应性逐渐降低。相比之下，从损伤后2 - 7天，这些变性轴突中的荧光玉染色逐渐增加。在损伤近端观察到用示踪剂荧光金和抗GAP43双重标记的芽中的再生。
本研究中使用的标准化视神经挤压伤模型导致了可重复的变性模式，包括明显的星形胶质细胞和小胶质细胞增殖。变性轴突中神经丝免疫反应性的逐渐降低以及荧光玉染色的逐渐增加与损伤近端的自发再生平行，通过示踪和GAP43免疫反应性显示。因此，这些标记物与该实验设置的组合允许在标准化的中枢神经系统轴突切断模型中分析整个变性/再生过程。
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