Layton J E, Johnson G R, Scott D W, Nossal G J
Eur J Immunol. 1978 May;8(5):325-30. doi: 10.1002/eji.1830080507.
The ontogeny of IgD was examined in neonatal to adult mice by immunofluorescence. The first delta-positive cells appeared in the spleen at about 3 days of age, while lymph nodes from 4 to 5-day-old mice already contained 10--20% delta-positive B cells. In order to study the function of IgD, we developed a system in which the appearance of delta-positive cells was suppressed by treatment of mice from birth with an anti-delta allotype serum. Immunofluorescence studies showed that very few delta-positive cells could be found in the spleen and lymph nodes of such suppressed mice, and also that the percentage of mu-positive cells was reduced by about 50%. The recovery from suppression was rapid, indicating that potential delta-positive cells were modulated rather than killed. Studies of the immune response of suppressed mice in vitro and in vivo showed that suppression had little effect on the IgM response but caused a definite reduction in the IgG response.
通过免疫荧光法检测了新生小鼠到成年小鼠体内IgD的个体发生情况。最早的δ阳性细胞大约在3日龄时出现在脾脏中，而4至5日龄小鼠的淋巴结中已经含有10%-20%的δ阳性B细胞。为了研究IgD的功能，我们建立了一个系统，在该系统中，从出生就用抗δ同种异型血清处理小鼠，从而抑制δ阳性细胞的出现。免疫荧光研究表明，在这种受抑制小鼠的脾脏和淋巴结中几乎找不到δ阳性细胞，而且μ阳性细胞的百分比也降低了约50%。抑制作用的恢复很快，这表明潜在的δ阳性细胞被调节而非被杀死。对受抑制小鼠在体外和体内的免疫反应研究表明，抑制作用对IgM反应影响很小，但导致IgG反应明显降低。
