Baine Y, Chen Y W, Jacobson E B, Pernis B, Siskind G W, Thorbecke G J
Eur J Immunol. 1982 Oct;12(10):882-6. doi: 10.1002/eji.1830121015.
Previous studies have shown that anti-IgD-suppressed mice give normal primary and secondary splenic plaque-forming cell responses following i.v. challenge, although mice suppressed by the injection of anti-IgD from birth lack IgD-bearing cells in all lymphoid tissue examined. The present studies show that, in contrast, secondary immune responses in regional lymph nodes of such mice, even after i.v. priming with trinitrophenylated B. abortus, respond to a challenge injection in the footpad up to only less than 10% of control levels. When compared with respect to B cell numbers transferred, primed spleen cells from control and anti-IgD-suppressed mice are about equally effective in producing adoptive secondary plaque-forming cell responses in the spleens of recipient mice. Lymph nodes in recipients of anti-IgD-suppressed primed spleen cells show much lower responses than do lymph nodes in recipients of control primed cells, both upon immediate and delayed challenge with antigen in the footpads. It is concluded that the immunodeficiency caused by suppression with anti-IgD is much more marked in peripheral lymph nodes than in the spleen. The possible relationship of these results to the migratory properties of IgD+ as compared to IgD-B cells is discussed.
先前的研究表明，抗IgD抑制的小鼠经静脉内攻击后，脾原发性和继发性斑块形成细胞反应正常，尽管从出生就注射抗IgD而被抑制的小鼠在所检查的所有淋巴组织中均缺乏携带IgD的细胞。相比之下，本研究表明，即使在用三硝基苯化牛流产布鲁氏菌进行静脉内致敏后，此类小鼠局部淋巴结中的二次免疫反应，对足垫中的激发注射的反应也仅达到对照水平的不到10%。就转移的B细胞数量而言，对照小鼠和抗IgD抑制小鼠的致敏脾细胞在受体小鼠脾脏中产生过继性继发性斑块形成细胞反应的效果大致相同。在用抗原立即和延迟攻击足垫时，抗IgD抑制的致敏脾细胞受体中的淋巴结反应比对照致敏细胞受体中的淋巴结反应低得多。得出的结论是，抗IgD抑制所导致的免疫缺陷在周围淋巴结中比在脾脏中更为明显。讨论了这些结果与IgD+细胞和IgD-细胞迁移特性相比的可能关系。
