Ah Fong A M V, Judelson H S
Department of Plant Pathology, University of California, Riverside, CA 92521, USA.
Mol Genet Genomics. 2004 Jun;271(5):577-85. doi: 10.1007/s00438-004-1004-x. Epub 2004 Apr 20.
A family of transposable elements belonging to the hAT group of DNA transposons is described from an oomycete, the plant pathogen Phytophthora infestans. The family, named DodoPi, was identified by studying a hotspot for retro- and DNA transposon insertions adjacent to the mating type locus. The DodoPi family comprises a small number of full-length copies, each of which is 2.7 kb long and predicted to encode a transposase-like protein consisting of 617 amino acids, and several truncated copies. Both types contain 12-bp terminal inverted repeats and are flanked by 8-bp target site duplications. Despite the detection of a DodoPi transcript and of many polymorphisms between isolates, conclusive evidence of recent transposition was not obtained. A phylogenetic analysis indicated that DodoPi was novel, with only modest similarity to some elements from plants and fungi. Relatives were detected in only some members of the genus. This is the first DNA transposon identified in the stramenopile group of eukaryotes.
从卵菌纲植物病原菌致病疫霉（Phytophthora infestans）中鉴定出一个属于DNA转座子hAT家族的转座元件家族。通过研究与交配型位点相邻的逆转录转座子和DNA转座子插入热点，鉴定出了这个名为多多皮（DodoPi）的家族。多多皮家族由少数全长拷贝组成，每个拷贝长2.7 kb，预计编码一种由617个氨基酸组成的转座酶样蛋白，以及几个截短的拷贝。这两种类型都含有12 bp的末端反向重复序列，并由8 bp的靶位点重复序列侧翼。尽管检测到了多多皮转录本以及分离株之间的许多多态性，但未获得近期转座的确凿证据。系统发育分析表明，多多皮是新发现的，与植物和真菌中的一些元件只有适度的相似性。仅在该属的一些成员中检测到了相关元件。这是在真核生物的不等鞭毛类群中鉴定出的第一个DNA转座子。
