Hammer T, Bode R
Institut für Biochemie, Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald.
Zentralbl Mikrobiol. 1992;147(1-2):65-70. doi: 10.1016/s0232-4393(11)80365-3.
L-Lysine epsilon-dehydrogenase [L-lysine:NADP+ oxidoreductase (epsilon-deaminating), EC 1.4.1.15] of Candida albicans was studied emphasizing its application for the production of alpha-aminoadipate-delta-semialdehyde and related compounds. A high enzyme level (240 pkat/mg of protein in the crude extract) could be attained during growth in the presence of L-lysine as sole nitrogen source. After optimization of the reaction conditions a partial purified enzyme (1.5 nkat/mg of protein) was used to produce alpha-aminoadipate-delta-semialdehyde, S-(beta-acetaldehyde)-cysteine, alpha-amino-delta-hydroxyadipate-semialdehyde and alpha-amino-gamma-hydroxyadipate-semialdehyde from the corresponding substrates lysine, S-(beta-aminoethyl)-cysteine, 5-hydroxylysine and 4-hydroxylysine, respectively. After purification of the compounds using Dowex 50 x 4 chromatography a yield of the products between 4.6 and 6.8% was achieved.
对白色念珠菌的L-赖氨酸ε-脱氢酶[L-赖氨酸：NADP⁺氧化还原酶（ε-脱氨基），EC 1.4.1.15]进行了研究，重点关注其在生产α-氨基己二酸-δ-半醛及相关化合物方面的应用。在以L-赖氨酸作为唯一氮源的生长过程中，可达到较高的酶水平（粗提物中为240 pkat/mg蛋白质）。在优化反应条件后，使用部分纯化的酶（1.5 nkat/mg蛋白质）分别从相应底物赖氨酸、S-（β-氨基乙基）-半胱氨酸、5-羟基赖氨酸和4-羟基赖氨酸生产α-氨基己二酸-δ-半醛、S-（β-乙醛基）-半胱氨酸、α-氨基-δ-羟基己二酸半醛和α-氨基-γ-羟基己二酸半醛。使用Dowex 50×4色谱法纯化化合物后，产物的产率达到4.6%至6.8%。
