Sterzik K, Schmid U, Strehler E, Rosenbusch B, Schill W B, Lauritzen C
Frauenklinik der Universität Ulm.
Zentralbl Gynakol. 1992;114(7):365-9.
A significant enhancement of the fertilizing capacity of human spermatozoa by Kallikrein has recently been described in a former study. To assess the therapeutic possibilities it would be of great interest to know more about the biochemical effect of Kallikrein on the fertilizing capacity of human spermatozoa. This fact made the authors investigate 64 semen samples in which the binding score and the fertilizing capacity were determined after application of Kallikrein in a HOP-test (hamsterovum-penetration-test) and compared to the control. Furthermore these results were compared to the varying concentrations of the components of the Kallikrein-Kinin-system. An application of Kallikrein in the swim-up preparation resulted in a higher motility in patients with asthenozoospermia, whereas the patients belonging to the group of oligoasthenozoospermia showed a lower progressive motility compared to the control. The binding score (number of affixed spermatozoa) showed nearly the results in both preparations. On the other hand the fertilizing capacity in the Kallikrein preparation was significantly higher. A possible connection with the concentrations of the components of seminal plasma has not been found, yet, but regarding all results a direct enzymatic effect of Kallikrein on fertilizing potential can be assumed.
最近在一项先前的研究中描述了激肽释放酶对人类精子受精能力的显著增强作用。为了评估治疗可能性，进一步了解激肽释放酶对人类精子受精能力的生化作用将非常有意义。基于此，作者研究了64份精液样本，在仓鼠卵穿透试验（HOP试验）中应用激肽释放酶后测定其结合评分和受精能力，并与对照组进行比较。此外，还将这些结果与激肽释放酶-激肽系统各成分的不同浓度进行了比较。在游动精子制备中应用激肽释放酶，弱精子症患者的精子活力有所提高，而少弱精子症组患者的前向运动能力与对照组相比有所降低。两种制备方法的结合评分（附着精子数量）结果相近。另一方面，激肽释放酶制备组的受精能力显著更高。目前尚未发现与精浆成分浓度之间的可能联系，但综合所有结果，可以推测激肽释放酶对受精潜能有直接的酶促作用。
