[来自土拉热弗朗西斯菌外膜的脂多糖-蛋白质复合物]

[The LPS-protein complex from the outer membrane of Francisella tularensis].

作者信息

Khlebnikov V S, Kulevatskiĭ D P, Golovlev I R, Averin S F, Zhemchugov V E, Chugunov A M, Afanas'ev S S

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1992 Mar(3):13-7.

Abstract

LPS-protein complex containing proteins of 15 kD, 17 kD and 19 kD was isolated from F. tularensis outer membrane by solving with sodium deoxycholate with the subsequent gel filtration on Sephacryl S-200. Protein of 17 kD constituted the main protein component of the complex. The LPS-protein ratio of this complex was 1:1. Proteins contained in LPS-protein complex have mainly the alpha-spiral structure. In the absence of detergent these proteins and LPS formed micelles with molecular weight exceeding 10(7) D. LPS-protein complex was shown to have a protective effect in mice infected with F. tularensis virulent strain 503.

摘要

通过用脱氧胆酸钠溶解，随后在Sephacryl S - 200上进行凝胶过滤，从土拉热弗朗西斯菌外膜中分离出含有15kD、17kD和19kD蛋白质的脂多糖 - 蛋白质复合物。17kD的蛋白质是该复合物的主要蛋白质成分。该复合物的脂多糖与蛋白质的比例为1:1。脂多糖 - 蛋白质复合物中所含的蛋白质主要具有α - 螺旋结构。在没有去污剂的情况下，这些蛋白质和脂多糖形成了分子量超过10⁷D的胶束。脂多糖 - 蛋白质复合物在感染土拉热弗朗西斯菌强毒株503的小鼠中显示出保护作用。