Khlebnikov V S, Golovlev I R, Kulevatskiĭ D P, Averin S F, Pshirkov S Iu, Tokhtamysheva N V, Zhemchugov V E, Safonova L A, Gerasimov V N, Chugunov A M
Mol Gen Mikrobiol Virusol. 1991 Jul(7):15-20.
The outer membranes of Francisella tularensis were studied. The membranes were identified morphologically, immunologically and biochemically. They contained 12-20% of protein, 15-30% of carbohydrates, up to 40% of lipids. The main integral proteins of the outer membranes were the 47, 43, 17 and 12 kD proteins. The main protein 63 kD was not integral. The lipopolysaccharides isolated from the outer membranes and acetone-dried cells did not possess the protective properties in experimental tularemia. The preparations of outer membranes possessed the protective properties for mice infected with the virulent strain 503. Chitosan amplified the protective properties of outer membranes.
对土拉弗朗西斯菌的外膜进行了研究。通过形态学、免疫学和生物化学方法对这些膜进行了鉴定。它们含有12% - 20%的蛋白质、15% - 30%的碳水化合物以及高达40%的脂质。外膜的主要整合蛋白是47、43、17和12 kD的蛋白质。主要的63 kD蛋白质不是整合蛋白。从外膜和丙酮干燥细胞中分离出的脂多糖在实验性兔热病中不具有保护特性。外膜制剂对感染强毒株503的小鼠具有保护特性。壳聚糖增强了外膜的保护特性。
