He Yonghong, Gao Zhixian, Chao Fuhuan
Institute of Hygiene and Environment Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Wei Sheng Yan Jiu. 2004 Jan;33(1):112-4.
The application of pesticide residue analysis techniques based on biological principle, such as immunoassay, biosensor and direct test the influence of pesticide upon the activity of the enzyme and the growth of the propagation, was reviewed in this paper. Immunoassay is a kind of precise and sensitive method, but it's not easy to produce the antibody of pesticide. The strongpoint of biosensor is the process of the detection is quickly and real time, but the sensitivity and stability of biosensor still need improve. The tests based on the activity of the enzyme and the growth of the propagation are simple and inexpensive, and are useful in the situation of acute-poisoning and severe contamination. These techniques general work fast, cost inexpensive, and do not need large-volume unit, so they have a good large potential in extensive pesticide residue monitoring and spot assays.
本文综述了基于生物学原理的农药残留分析技术的应用，如免疫测定、生物传感器以及直接检测农药对酶活性和繁殖生长的影响。免疫测定是一种精确且灵敏的方法，但制备农药抗体并不容易。生物传感器的优点是检测过程快速且实时，但其灵敏度和稳定性仍有待提高。基于酶活性和繁殖生长的检测方法简单且成本低廉，适用于急性中毒和严重污染的情况。这些技术通常工作速度快、成本低，且不需要大型设备，因此在广泛的农药残留监测和现场检测中具有很大的潜力。
