Nishijima M
Tokyo Metropolitan Research Laboratory of Public Health.
Nihon Rinsho. 1995 Sep;53(9):2310-5.
Analytical techniques for pesticide residues in food include gas chromatography (GC), gas chromatography/mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC). These procedures are time consuming, cumbersome and require specialized instrumentation. A number of enzyme immunoassay methods for pesticide have appeared in recent years. In addition, nowadays a lot of immunoassay kits for various kinds of pesticide are in the market. The assay offers alternative means that is more sensitive, faster and cost-effective than previous methods. However, it is subject to problems that the assay does not suit multi-residue analysis and has possibility to encounter interferences which would affect the assay precision in case of some crops. Assuming that researcher knows which pesticide were applied to the analyzed crops beforehand, the pesticide would be detected easily by immunoassay. Also, when suitable sample kit for analyzed pesticide is available, the assay could be an excellent screening tool for large number of food samples. In conclusion, it will be necessary to consider analytical purpose to confirm utility of immunoassay when applying to pesticide analysis.
食品中农药残留的分析技术包括气相色谱法(GC)、气相色谱/质谱联用法(GC-MS)和高效液相色谱法(HPLC)。这些方法耗时、繁琐且需要专门的仪器设备。近年来出现了许多用于农药检测的酶免疫分析方法。此外,如今市场上有许多针对各类农药的免疫分析试剂盒。该分析方法提供了比以往方法更灵敏、快速且经济高效的替代手段。然而,它存在一些问题,即该分析方法不适用于多残留分析,并且在某些作物的情况下有可能遇到会影响分析精度的干扰。假设研究人员事先知道哪些农药施用于所分析的作物,那么通过免疫分析就可以轻松检测出这些农药。而且,当有适用于所分析农药的合适样品试剂盒时,该分析方法可以成为大量食品样品的出色筛选工具。总之,在将免疫分析应用于农药分析时,有必要考虑分析目的以确认其效用。