Yamada Takenori, Kunioka Yuki, Wakayama Jun'ichi, Aimi Momoko, Noguchi Yu-suke, Akiyama Nao, Kayamori Taisuke
Department of Physics (Biophysics Section), Faculty of Science, Science University of Tokyo, Shinjuku-ku, Tokyo 162-8601, Japan.
Adv Exp Med Biol. 2003;538:285-94; discussion 294. doi: 10.1007/978-1-4419-9029-7_27.
By applying AFM technology, we studied mechanical characteristics of myofibrils of skeletal muscle. The obtained results indicate that (1) the Z-band is the most rigid sarcomere component stabilizing the myofibril structures, (2) various filamentous components are inter-connected in sarcomere with sufficient mechanical strength to support the contractile force, and (3) the molecular structure of the overlap region between actin and myosin filaments is anisotropic. In any case the present studies clearly indicate that the AFM technique is a powerful tool to investigate the mechanical characteristics of sarcomere structure of muscle fiber.
通过应用原子力显微镜（AFM）技术，我们研究了骨骼肌肌原纤维的力学特性。所得结果表明：（1）Z线是稳定肌原纤维结构的最刚性的肌节成分；（2）肌节中的各种丝状成分以足够的机械强度相互连接，以支撑收缩力；（3）肌动蛋白丝和肌球蛋白丝重叠区域的分子结构是各向异性的。无论如何，目前的研究清楚地表明，AFM技术是研究肌纤维肌节结构力学特性的有力工具。
