Taniguchi Tomohiro, Torii Toru, Higuchi Toshiro
Department of Precision Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8656, Japan.
Lab Chip. 2002 Feb;2(1):19-23. doi: 10.1039/b108739h. Epub 2002 Jan 22.
A microchemical reaction method involving microdroplets is proposed. Microdroplets are formed in a chemically stable medium on electric panel devices. These devices are substrates which have electrode arrays or electrode dots, and its surfaces are coated by an insulating film (such as Teflon or polypropylene) to prevent discharge and electrolysis of solutions. Microdroplets can be separately manipulated by a traveling electric field, which arises on applying a sequential voltage to the electrodes. Droplets moved smoothly at 1 Hz and voltage 400 V(0-p). Reagents were then put in droplets that were collided and coalesced, resulting in chemical reactions that included alkalization of phenolphthalein and the luciferin-luciferase reaction.
