Davis Alen N, Samlali Kenza, Kapadia Jay B, Perreault Jonathan, Shih Steve C C, Kharma Nawwaf
Department of Electrical and Computer Engineering, Concordia University, Montreal, Québec H3G 1M8, Canada.
Centre for Applied Synthetic Biology, Concordia University, Montréal, Québec H4B 1R6, Canada.
ACS Omega. 2021 Aug 25;6(35):22514-22524. doi: 10.1021/acsomega.1c00239. eCollection 2021 Sep 7.
In this paper, we describe the design and performance of two digital microfluidics (DMF) chips capable of executing multiple ribozymatic reactions, with proper controls, in response to short single-stranded DNA inducers. Since the fluorescence output of a reaction is measurable directly from the chip, without the need for gel electrophoresis, a complete experiment involving up to eight reactions (per chip) can be carried out reliably, relatively quickly, and efficiently. The ribozymes can also be used as biosensors of the concentration of oligonucleotide inputs, with high sensitivity, low limits of quantification and of detection, and excellent signal-to-noise ratio. The presented chips are readily usable devices that can be used to automate, speed up, and reduce the costs of ribozymatic reaction experiments.
在本文中，我们描述了两种数字微流控（DMF）芯片的设计与性能。这两种芯片能够响应短单链DNA诱导剂，在适当的控制下执行多个核酶反应。由于反应的荧光输出可直接从芯片上测量，无需进行凝胶电泳，因此涉及多达八个反应（每个芯片）的完整实验能够可靠、相对快速且高效地进行。这些核酶还可用作寡核苷酸输入浓度的生物传感器，具有高灵敏度、低定量限和检测限以及出色的信噪比。所展示的芯片是易于使用的设备，可用于实现核酶反应实验的自动化、加快实验速度并降低成本。
