Binz Pierre-Alain, Müller Markus, Hoogland Christine, Zimmermann Catherine, Pasquarello Carla, Corthals Garry, Sanchez Jean-Charles, Hochstrasser Denis F, Appel Ron D
Swiss Institute of Bioinformatics, Proteome Informatics Group, CMU, Michel Servet 1, 1211 Geneva, Switzerland.
Curr Opin Biotechnol. 2004 Feb;15(1):17-23. doi: 10.1016/j.copbio.2003.12.003.
Approaches aimed at deciphering the proteome have illustrated the need for relatively complex and highly sensitive methodologies. The major elements of proteome analysis, such as powerful protein separation and enzymatic processing, mass spectrometry and dedicated bioinformatics have been assembled in the development of the molecular scanner. This highly flexible and data-rich approach has combined the power of electrophoretic protein separation, the simultaneous digestion and transfer of proteins through an enzymatic membrane, the immediate use of the MALDI mass spectrometer to scan a collecting membrane, and the development of dedicated bioinformatics tools to perform protein identification and molecular imaging of the proteome. Clinical applications of the molecular scanner have also started to be developed for disease diagnosis in biological material.
旨在解析蛋白质组的方法已经表明需要相对复杂且高度灵敏的方法。蛋白质组分析的主要要素，如强大的蛋白质分离和酶处理、质谱分析以及专用的生物信息学，都被整合到了分子扫描仪的开发中。这种高度灵活且数据丰富的方法结合了电泳蛋白质分离的能力、通过酶膜同时进行蛋白质消化和转移、立即使用基质辅助激光解吸电离质谱仪扫描收集膜，以及开发专用的生物信息学工具来进行蛋白质鉴定和蛋白质组的分子成像。分子扫描仪的临床应用也已开始用于生物材料中的疾病诊断。
