Kippen I, Nakata N, Klinenberg J R
J Pharmacol Exp Ther. 1977 Apr;201(1):218-25.
A rapid filtration procedure was used to determine rates of uric acid uptake by a preparation of separated renal cortical tubules of the rabbit. The rate of uric acid uptake was temperature dependent and showed saturation kinetics with a K of 3.2 mM. The uptake was 50% lower when measured under nitrogen as compared with uptake under oxygen. The uptake rate increased with increasing sodium concentration and decreased with increasing potassium concentration. Uptake was stimulated by citrate, succinate and pyruvate, and inhibited by alpha-ketoglutarate. Parathyroid hormone and 10(-4) M adenosine 3':5'-monophosphate increased the uric acid uptake rate when preincubated with the tubules for 130 minutes before the addition of uric acid. Uric acid uptake in this preparation appears to occur by some form of carrier-mediated active transport.
采用快速过滤程序来测定兔离体肾皮质小管制剂对尿酸的摄取速率。尿酸摄取速率取决于温度，并呈现饱和动力学，其K值为3.2 mM。与在氧气条件下测量的摄取量相比，在氮气条件下测量时摄取量降低了50%。摄取速率随钠浓度增加而升高，随钾浓度增加而降低。柠檬酸盐、琥珀酸盐和丙酮酸盐可刺激摄取，而α-酮戊二酸则抑制摄取。甲状旁腺激素和10(-4) M 3':5'-环磷酸腺苷在加入尿酸前与小管预孵育130分钟时，可提高尿酸摄取速率。该制剂中的尿酸摄取似乎是通过某种形式的载体介导的主动转运发生的。
