Calonge Margarita, Diebold Yolanda, Sáez Victoria, Enríquez de Salamanca Amalia, García-Vázquez Carmen, Corrales Rosa M, Herreras José M
Instituto Universitario de Oftalmobiología Aplicada (IOBA), University of Valladolid, Ramón y Cajal, 7, E-47005 Valladolid, Spain.
Exp Eye Res. 2004 Mar;78(3):457-72. doi: 10.1016/j.exer.2003.09.009.
To historically review the technique of impression cytology as a minimally invasive diagnostic tool for ocular surface pathology.
A comprehensive review of published literature cited in PubMed since the first description of impression cytology in 1977 up to date has been undertaken.
A wide range of processing methods have been adapted to the technique of impression cytology (from conjunctiva, cornea or limbus): regular light microscopy with different stainings, transmission and scanning electron microscopy, immunofluorescence, immunocytochemistry, polymerase chain reaction analysis, immunoblotting analyses, or flow cytometry. At present, it is widely used as a non-invasive alternative to the full-thickness biopsy for the obtention of epithelial cells from the ocular surface.
Impression cytology represents a non- or minimally invasive biopsy of the ocular surface epithelium with no side effects or contraindications. It has demonstrated to be a useful diagnostic aid for a wide variety of processes involving the ocular surface. In addition, and mainly during the last decade, its use as a research tool has experienced an enormous growth and has greatly contributed to the understanding of ocular surface pathology.
从历史角度回顾印片细胞学技术作为眼表病理学微创诊断工具的情况。
对自1977年首次描述印片细胞学技术至今在PubMed上引用的已发表文献进行全面综述。
印片细胞学技术（取自结膜、角膜或角膜缘）已采用多种处理方法：不同染色的常规光学显微镜检查、透射和扫描电子显微镜检查、免疫荧光、免疫细胞化学、聚合酶链反应分析、免疫印迹分析或流式细胞术。目前，它被广泛用作从眼表获取上皮细胞的全层活检的非侵入性替代方法。
印片细胞学是眼表上皮的非侵入性或微创活检方法，无副作用或禁忌证。它已被证明对涉及眼表的多种病症是一种有用的诊断辅助手段。此外，主要在过去十年中，其作为研究工具的应用有了巨大增长，并极大地促进了对眼表病理学的理解。
