用于灵敏检测水痘-带状疱疹病毒特异性IgG的免疫荧光试验：膜抗原荧光抗体试验的替代方法

Immunofluorescence test for sensitive detection of varicella-zoster virus-specific IgG: an alternative to fluorescent antibody to membrane antigen test.

作者信息

Sauerbrei A, Färber I, Brandstädt A, Schacke M, Wutzler P

机构信息

Institute of Virology and Antiviral Therapy, Friedrich-Schiller University of Jena, Postfach, D-07740 Jena, Germany.

出版信息

J Virol Methods. 2004 Jul;119(1):25-30. doi: 10.1016/j.jviromet.2004.02.012.

DOI:10.1016/j.jviromet.2004.02.012

PMID:15109817

Abstract

A highly sensitive indirect fluorescence antibody test (IFAT) has been developed on the basis of varicella-zoster virus (VZV)-infected human lung carcinoma (A549) cells and evaluated for the determination of immunity to VZV. Different serum panels with negative, low, moderate or high anti-VZV IgG levels detected by the fluorescent antibody to membrane antigen (FAMA) assay were investigated. As a result, the sensitivity and the specificity of IFAT were 100% compared to FAMA test. In anti-VZV IgG-positive sera, a significant correlation between the results of FAMA procedure and IFAT could be shown. However, there were considerably higher antibody titers by the IFAT than by FAMA. Whereas the FAMA test had a detection limit of 250 mIU/ml anti-VZV IgG, the limit of detection of IFAT was 50 mIU/ml. In conclusion, the IFAT using VZV-infected A549 cells as antigen allows a highly sensitive, specific, and rapid detection of anti-VZV IgG class antibodies. This simple technique can replace the labor-intensive FAMA procedure for laboratory determination of immunity to VZV.

摘要

基于水痘带状疱疹病毒（VZV）感染的人肺癌（A549）细胞，开发了一种高灵敏度间接荧光抗体试验（IFAT），并对其在确定VZV免疫力方面进行了评估。研究了通过膜抗原荧光抗体（FAMA）检测法检测出的具有阴性、低、中或高抗VZV IgG水平的不同血清组。结果显示，与FAMA试验相比，IFAT的灵敏度和特异性均为100%。在抗VZV IgG阳性血清中，FAMA检测结果与IFAT结果之间存在显著相关性。然而，IFAT检测出的抗体滴度明显高于FAMA。FAMA试验的抗VZV IgG检测限为250 mIU/ml，而IFAT的检测限为50 mIU/ml。总之，以VZV感染的A549细胞为抗原的IFAT能够高度灵敏、特异且快速地检测抗VZV IgG类抗体。这种简单的技术可以替代用于实验室测定VZV免疫力的劳动强度大的FAMA检测法。