蛋白激酶在溶组织内阿米巴红细胞吞噬过程中的作用。

Involvement of protein kinases on the process of erythrophagocytis by Entamoeba histolytica.

作者信息

Batista Evander de J O, de Souza Wanderley

机构信息

Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS-Bloco G, Ilha do Fundão, Rio de Janeiro RJ, 21941-900, Brazil.

出版信息

Cell Biol Int. 2004;28(4):243-8. doi: 10.1016/j.cellbi.2004.01.005.

DOI:10.1016/j.cellbi.2004.01.005

PMID:15109979

Abstract

Erythrophagocytic capacity of trophozoites of Entamoeba histolytica is considered a factor in the virulence of this pathogenic protozoan. We present evidence showing that such activity resembles the ingestion of microorganisms by highly differentiated phagocytic cells, such as macrophages. Previous treatment of the trophozoites with genistein or tyrphostin, inhibitors of tyrosine protein kinases, with staurosporine, a protein kinase C inhibitor, and wortmannin, a fungal metabolite that inhibits phosphoinositide 3-OH kinase, significantly inhibited their erythrophagocytic capacity.

摘要

溶组织内阿米巴滋养体的红细胞吞噬能力被认为是这种致病性原生动物毒力的一个因素。我们提供的证据表明，这种活性类似于高度分化的吞噬细胞（如巨噬细胞）对微生物的摄取。用酪氨酸蛋白激酶抑制剂染料木黄酮或 tyrphostin、蛋白激酶 C 抑制剂星形孢菌素以及抑制磷酸肌醇 3-OH 激酶的真菌代谢产物渥曼青霉素预先处理滋养体，可显著抑制其红细胞吞噬能力。