Munguía-Robledo Susana, Orozco Esther, García-Rivera Guillermina, Bolaños Jeni, Valdés Jesús, Azuara-Licéaga Elisa, Rodríguez Mario Alberto
Center for Research and Advanced Studies of the IPN, Department of Infectomics and Molecular Pathogenesis, National Polytechnic Institute, Mexico City 07360, Mexico.
Center for Research and Advanced Studies of the IPN, Department of Biochemistry, National Polytechnic Institute, Mexico City 07360, Mexico.
Pathogens. 2023 Mar 17;12(3):474. doi: 10.3390/pathogens12030474.
Lysine methylation, a posttranslational modification catalyzed by protein lysine methyltransferases (PKMTs), is involved in epigenetics and several signaling pathways, including cell growth, cell migration and stress response, which in turn may participate in virulence of protozoa parasites. , the etiologic agent of human amebiasis, has four PKMTs (EhPKMT1 to EhPKMT4), but their role in parasite biology is unknown. Here, to obtain insight into the role of EhPKMT2, we analyzed its expression level and localization in trophozoites subjected to heat shock and during phagocytosis, two events that are related to amoeba virulence. Moreover, the effect of EhPKMT2 knockdown on those activities and on cell growth, migration and cytopathic effect was investigated. The results indicate that this enzyme participates in all these cellular events, suggesting that it could be a potential target for development of novel therapeutic strategies against amebiasis.
赖氨酸甲基化是一种由蛋白质赖氨酸甲基转移酶（PKMTs）催化的翻译后修饰，参与表观遗传学和多种信号通路，包括细胞生长、细胞迁移和应激反应，这些反过来可能参与原生动物寄生虫的毒力。溶组织内阿米巴，人类阿米巴病的病原体，有四种PKMTs（EhPKMT1至EhPKMT4），但其在寄生虫生物学中的作用尚不清楚。在这里，为了深入了解EhPKMT2的作用，我们分析了其在热休克处理的滋养体以及吞噬过程中的表达水平和定位，这两个事件与变形虫的毒力有关。此外，还研究了EhPKMT2敲低对这些活动以及细胞生长、迁移和细胞病变效应的影响。结果表明，这种酶参与了所有这些细胞事件，表明它可能是开发针对阿米巴病的新型治疗策略的潜在靶点。
