利用单克隆抗体对马尔尼菲青霉菌进行免疫组织化学鉴定。

Immunohistochemical identification of Penicillium marneffei by monoclonal antibody.

作者信息

Arrese Estrada J, Stynen D, Van Cutsem J, Piérard-Franchimont C, Piérard G E

机构信息

Department of Dermatopathology, CHU du Sart Tilman, Liège, Belgium.

出版信息

Int J Dermatol. 1992 Jun;31(6):410-2. doi: 10.1111/j.1365-4362.1992.tb02670.x.

DOI:10.1111/j.1365-4362.1992.tb02670.x

PMID:1512093

Abstract

We present an experimental study on the immunohistochemical identification of Penicillium marneffei in paraffin-embedded, formalin-fixed tissue. The monoclonal antibody EB-A1 detects a specific galactomannan that appears to have at least one epitope identical in P. marneffei and Aspergillus sp. This immunohistochemical approach could be useful in the diagnosis of a rare diseae, penicilliosis marneffei, which proves to be difficult to identify by conventional microscopy.

摘要

我们展示了一项关于在福尔马林固定、石蜡包埋组织中对马尔尼菲青霉菌进行免疫组织化学鉴定的实验研究。单克隆抗体EB-A1可检测到一种特定的半乳甘露聚糖，该半乳甘露聚糖似乎在马尔尼菲青霉菌和曲霉属中至少有一个相同的表位。这种免疫组织化学方法可能有助于诊断一种罕见疾病——马尔尼菲青霉病，事实证明，通过传统显微镜检查很难对其进行鉴定。