Łuczaj Wojciech, Waszkiewicz Ewa, Skrzydlewska Elzbieta, Roszkowska-Jakimiec Wiesława
Department of Analytical Chemistry, Medical University of Białystok, Białystok, Poland.
J Toxicol Environ Health A. 2004 Apr 9;67(7):595-606. doi: 10.1080/15287390490425579.
Ethanol intoxication leads to oxidative stress, which may be additionally enhanced by aging. The aim of this study was to investigate the influence of green tea as a source of water-soluble antioxidants on the ability to prevent oxidative stress in aged rats sub-chronically intoxicated with ethanol. Two-, 12-, and 24-mo-old male Wistar rats were divided into 4 experimental groups: (1) control, (2) green tea, (3) ethanol, and (4) ethanol and green tea. Ethanol intoxication produced age-dependent decrease in the activity of serum superoxide dismutase, glutathione peroxidase, and reductase and in levels of glutathione (GSH), vitamins C, E, and A, and beta-carotene. Changes in the serum antioxidative ability were accompanied by enhanced oxidative modification of lipid (increase in lipid hydroperoxides, malondiadehyde, and 4-hydroxynonenal levels) and protein (rise in carbonyl group levels). Green tea partially protected against changes in antioxidant enzymatic as well as nonenzymatic parameters produced by ethanol and enhanced by aging. Administration of green tea significantly protects cellular components such as lipids and proteins against oxidative modification. Results indicate that green tea effectively protects blood serum against oxidative stress produced by ethanol as well as aging.
乙醇中毒会导致氧化应激，而衰老可能会进一步加剧这种应激。本研究的目的是探究作为水溶性抗氧化剂来源的绿茶对预防亚慢性乙醇中毒老龄大鼠氧化应激能力的影响。将2月龄、12月龄和24月龄的雄性Wistar大鼠分为4个实验组：（1）对照组，（2）绿茶组，（3）乙醇组，（4）乙醇加绿茶组。乙醇中毒导致血清超氧化物歧化酶、谷胱甘肽过氧化物酶和还原酶的活性以及谷胱甘肽（GSH）、维生素C、E、A和β-胡萝卜素水平出现与年龄相关的下降。血清抗氧化能力的变化伴随着脂质（脂质氢过氧化物、丙二醛和4-羟基壬烯醛水平升高）和蛋白质（羰基水平升高）氧化修饰的增强。绿茶部分保护了由乙醇引起并因衰老而加剧的抗氧化酶和非酶参数的变化。给予绿茶可显著保护细胞成分如脂质和蛋白质免受氧化修饰。结果表明，绿茶能有效保护血清免受乙醇和衰老产生的氧化应激。
