Lin Yun, Yan Xiyun, Cao Wuchun, Wang Chooying, Feng Jing, Duan Jinzhu, Xie Sishen
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Antivir Ther. 2004 Apr;9(2):287-9.
A novel coronavirus, SARS-CoV, has been confirmed to be the aetiological agent of SARS. Transmission electron microscope (TEM) images played an important role in initial identification of the pathogen. In order to obtain greater morphological detail of SARS-CoV than could be obtained by TEM, we used ultra-high resolution scanning electron microscopy (SEM) to image the virus particles. We show here the three-dimensional appearance of SARS-CoV. Enhanced detail of the ultrastructure reveals the trimeric structure of the 10-20 nm spikes on the virion surface. These results contribute to characterization of the SARS agent and development of new antiviral strategies.
一种新型冠状病毒，即严重急性呼吸综合征冠状病毒（SARS-CoV），已被确认为严重急性呼吸综合征（SARS）的病原体。透射电子显微镜（TEM）图像在该病原体的初步鉴定中发挥了重要作用。为了获得比TEM所能提供的关于SARS-CoV更详细的形态细节，我们使用超高分辨率扫描电子显微镜（SEM）对病毒颗粒进行成像。我们在此展示了SARS-CoV的三维外观。超微结构细节的增强揭示了病毒粒子表面10 - 20纳米刺突的三聚体结构。这些结果有助于SARS病原体的特征描述以及新抗病毒策略的开发。
