Fehr Marcus, Ehrhardt David W, Lalonde Sylvie, Frommer Wolf B
Carnegie Institution of Washington, Plant Biology, 260 Panama Street, Stanford, California 94305, USA.
Curr Opin Plant Biol. 2004 Jun;7(3):345-51. doi: 10.1016/j.pbi.2004.03.015.
By 2010, it is expected that biochemical functions will be assigned to many of the products of the approximately 30,000 Arabidopsis genes. Moreover, systematic analysis of mutants will provide insight into the biological function of the gene products. Metabolomic technologies complement these approaches by testing for changes in cellular ion and metabolite patterns, providing essential information for the construction of cellular and whole-plant models of metabolism. However, one important set of information that is especially relevant for multicellular organisms is still lacking, that is, knowledge of the cellular and subcellular variation in metabolite levels. The recent development of protein-based nanosensors for metabolites will help to close this gap by providing a set of tools that can be used to determine cytosolic and subcellular metabolite levels in real time using fluorescence-based microscopy. A major challenge for the future is the application of these nanosensors to quantify metabolite levels in plant cells and tissues.
到2010年，预计拟南芥约30000个基因中的许多基因产物的生化功能将得以确定。此外，对突变体的系统分析将有助于深入了解基因产物的生物学功能。代谢组学技术通过检测细胞离子和代谢物模式的变化来补充这些方法，为构建细胞和全株代谢模型提供重要信息。然而，对于多细胞生物而言，仍缺少一组特别重要的信息，即代谢物水平在细胞和亚细胞层面的变化情况。最近开发的基于蛋白质的代谢物纳米传感器将有助于填补这一空白，它提供了一组工具，可用于通过基于荧光的显微镜实时测定胞质和亚细胞代谢物水平。未来的一个主要挑战是应用这些纳米传感器来量化植物细胞和组织中的代谢物水平。
