在植物中使用基因编码荧光生物传感器的展望。
Perspectives for using genetically encoded fluorescent biosensors in plants.
机构信息
Transport Biology Section, Department of Plant and Environmental Sciences, University of Copenhagen Copenhagen, Denmark.
出版信息
Front Plant Sci. 2013 Jul 12;4:234. doi: 10.3389/fpls.2013.00234. eCollection 2013.
Abstract
Genetically encoded fluorescent biosensors have long proven to be excellent tools for quantitative live imaging, but sensor applications in plants have been lacking behind those in mammalian systems with respect to the variety of sensors and tissue types used. How can this be improved, and what can be expected for the use of genetically encoded fluorescent biosensors in plants in the future? In this review, we present a table of successful physiological experiments in plant tissue using fluorescent biosensors, and draw some conclusions about the specific challenges plant cell biologists are faced with and some of the ways they have been overcome so far.
摘要
遗传编码荧光生物传感器长期以来一直被证明是定量活细胞成像的优秀工具，但就使用的传感器种类和组织类型而言，植物中的传感器应用落后于哺乳动物系统。如何改进这一点，以及在未来植物中使用遗传编码荧光生物传感器可以期待什么？在这篇综述中，我们提供了一个使用荧光生物传感器在植物组织中进行成功生理实验的表格，并就植物细胞生物学家面临的具体挑战以及迄今为止他们克服这些挑战的一些方法得出了一些结论。
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本文引用的文献
1
Organelle pH in the Arabidopsis endomembrane system.
Mol Plant. 2013 Sep;6(5):1419-37. doi: 10.1093/mp/sst079. Epub 2013 May 23.
2
A9C sensitive Cl⁻-accumulation in A. thaliana root cells during salt stress is controlled by internal and external calcium.
Plant Signal Behav. 2013 Jun;8(6):e24259. doi: 10.4161/psb.24259. Epub 2013 Apr 19.
3
High-resolution imaging of Ca2+ , redox status, ROS and pH using GFP biosensors.
Plant J. 2012 Apr;70(1):118-28. doi: 10.1111/j.1365-313X.2012.04917.x.
4
Quantitative imaging using genetically encoded sensors for small molecules in plants.
Plant J. 2012 Apr;70(1):108-17. doi: 10.1111/j.1365-313X.2012.04910.x.
5
Live imaging of intra- and extracellular pH in plants using pHusion, a novel genetically encoded biosensor.
J Exp Bot. 2012 May;63(8):3207-18. doi: 10.1093/jxb/ers040. Epub 2012 Mar 9.
6
Quantitative imaging with fluorescent biosensors.
Annu Rev Plant Biol. 2012;63:663-706. doi: 10.1146/annurev-arplant-042110-103745. Epub 2012 Feb 13.
7
New technologies for 21st century plant science.
Plant Cell. 2012 Feb;24(2):374-94. doi: 10.1105/tpc.111.093302. Epub 2012 Feb 24.
8
A novel sensor to map auxin response and distribution at high spatio-temporal resolution.
Nature. 2012 Jan 15;482(7383):103-6. doi: 10.1038/nature10791.
9
A toolset of aequorin expression vectors for in planta studies of subcellular calcium concentrations in Arabidopsis thaliana.
J Exp Bot. 2012 Feb;63(4):1751-61. doi: 10.1093/jxb/err406. Epub 2012 Jan 2.
10
The RootChip: an integrated microfluidic chip for plant science.
Plant Cell. 2011 Dec;23(12):4234-40. doi: 10.1105/tpc.111.092577. Epub 2011 Dec 20.
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