Transport Biology Section, Department of Plant and Environmental Sciences, University of Copenhagen Copenhagen, Denmark.
Front Plant Sci. 2013 Jul 12;4:234. doi: 10.3389/fpls.2013.00234. eCollection 2013.
Genetically encoded fluorescent biosensors have long proven to be excellent tools for quantitative live imaging, but sensor applications in plants have been lacking behind those in mammalian systems with respect to the variety of sensors and tissue types used. How can this be improved, and what can be expected for the use of genetically encoded fluorescent biosensors in plants in the future? In this review, we present a table of successful physiological experiments in plant tissue using fluorescent biosensors, and draw some conclusions about the specific challenges plant cell biologists are faced with and some of the ways they have been overcome so far.
遗传编码荧光生物传感器长期以来一直被证明是定量活细胞成像的优秀工具，但就使用的传感器种类和组织类型而言，植物中的传感器应用落后于哺乳动物系统。如何改进这一点，以及在未来植物中使用遗传编码荧光生物传感器可以期待什么？在这篇综述中，我们提供了一个使用荧光生物传感器在植物组织中进行成功生理实验的表格，并就植物细胞生物学家面临的具体挑战以及迄今为止他们克服这些挑战的一些方法得出了一些结论。
