鬼笔环肽和茉莉酮酸酯对肌动蛋白丝柔韧性和热稳定性的影响。
The effect of phalloidin and jasplakinolide on the flexibility and thermal stability of actin filaments.
作者信息
Visegrády Balázs, Lorinczy Dénes, Hild Gábor, Somogyi Béla, Nyitrai Miklós
机构信息
Department of Biophysics, Faculty of Medicine, University of Pécs, Szigeti str. 12, Pécs H-7624, Hungary.
出版信息
FEBS Lett. 2004 May 7;565(1-3):163-6. doi: 10.1016/j.febslet.2004.03.096.
In this work the effect of phalloidin and jasplakinolide on the dynamic properties and thermal stability of actin filaments was studied. Temperature dependent fluorescence resonance energy transfer measurements showed that filaments of Ca-actin became more rigid in the presence of phalloidin or jasplakinolide. Differential scanning calorimetric data implied that the stiffer filaments also had greater thermal stability in the presence of phalloidin or jasplakinolide. The fluorescence and calorimetric measurements provided evidences that the extent of stabilization by jasplakinolide was greater than that by phalloidin.
在这项工作中，研究了鬼笔环肽和茉莉素内酯对肌动蛋白丝动态特性和热稳定性的影响。温度依赖性荧光共振能量转移测量表明，在鬼笔环肽或茉莉素内酯存在下，钙肌动蛋白丝变得更加刚性。差示扫描量热数据表明，在鬼笔环肽或茉莉素内酯存在下，更硬的丝也具有更高的热稳定性。荧光和量热测量提供了证据，表明茉莉素内酯的稳定程度大于鬼笔环肽。
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