三种水通道蛋白基因胎盘表达的定量比较

Quantitative comparison of placental expression of three aquaporin genes.

作者信息

Liu H, Koukoulas I, Ross M C, Wang S, Wintour E M

机构信息

Department of Medical Genetics, Sun Yatsen University, China.

出版信息

Placenta. 2004 Jul;25(6):475-8. doi: 10.1016/j.placenta.2003.10.008.

DOI:10.1016/j.placenta.2003.10.008

PMID:15135229

Abstract

Three water channel proteins, aquaporins, have been shown to be expressed in the placentae of humans and sheep-AQP1, 3, 8; AQP1 is in the vasculature, whereas AQP3, 8 are in the trophoblast cells. In this study we used the sensitive and reproducible technique of real-time PCR to compare the level of expression of the mRNAs for AQP1, 3 and 8 in the ovine placenta at five stages of gestation (27, 45, 66, 100 and 140 days-where term approximately 150 days). AQP3 was quantitatively the most highly expressed AQP at 66, 100, and 140 days). At 27 days before significant trophoblast development had occurred, the only AQP present was AQP1, in the vasculature. The expression of these aquaporins underlie the high water and urea permeability of the ovine placenta in the last half of pregnancy.

摘要

已证实三种水通道蛋白在人类和绵羊的胎盘组织中均有表达，即水通道蛋白1、3、8；水通道蛋白1存在于血管系统中，而水通道蛋白3和8存在于滋养层细胞中。在本研究中，我们运用灵敏且可重复的实时聚合酶链反应技术，比较了妊娠五个阶段（27天、45天、66天、100天和140天，足月约为150天）绵羊胎盘组织中，水通道蛋白1、3和8的信使核糖核酸的表达水平。在妊娠66天、100天和140天时，水通道蛋白3的定量表达是最高的。在滋养层显著发育之前的27天，血管系统中唯一存在的水通道蛋白是水通道蛋白1。这些水通道蛋白的表达是妊娠后半期绵羊胎盘具有高水和尿素通透性的基础。