Clara Aranha, Manjramkar Dhananjay D, Reddy Venkatarami K
Immunology Laboratory, National Institute for Research in Reproductive Health, Parel, Mumbai, India.
Fertil Steril. 2004 May;81(5):1357-65. doi: 10.1016/j.fertnstert.2003.09.073.
To evaluate the safety and contraceptive efficacy of magainin-A in monkeys.
Controlled laboratory study.
Department of Immunology, National Institute for Research in Reproductive Health, Parel, Mumbai, India.
ANIMAL(S): Male and female bonnet monkeys (Macaca radiata).
INTERVENTION(S): Animals were treated intravaginally with 1 mg of magainin-A before attempted conception, as well as daily for 14 days to assess local and systemic toxicity.
MAIN OUTCOME MEASURE(S): Suitability of magainin-A for the control of pregnancy and sexually transmitted infections.
RESULT(S): Complete sperm immobilization was observed within 20 seconds after the exposure to magainin-A (800-1,000 microg) in vitro. Intravaginal administration of 1 mg of magainin-A blocked conception in monkeys. When magainin-A was administered intravaginally for 14 consecutive days, no treatment-related abnormalities were observed in menstrual cycle length, vaginal epithelial cell morphology, and hematologic/serum biochemical profiles. The peptide inhibited the growth of sexually transmitted infection-causing pathogens but not HIV-1 and HIV-2.
CONCLUSION(S): Magainin-A can be used as an effective and safe intravaginal contraceptive compound with additional protection against sexually transmitted infection-causing pathogens.
评估麦加宁 - A在猴子身上的安全性和避孕效果。
对照实验室研究。
印度孟买帕雷尔生殖健康国家研究所免疫学系。
成年帽猴（食蟹猴）。
在尝试受孕前，给动物阴道内给药1毫克麦加宁 - A，并连续14天每日给药，以评估局部和全身毒性。
麦加宁 - A对控制妊娠和性传播感染的适用性。
在体外，暴露于麦加宁 - A（800 - 1000微克）后20秒内观察到精子完全失活。阴道内给予1毫克麦加宁 - A可阻止猴子受孕。当连续14天阴道内给予麦加宁 - A时，在月经周期长度、阴道上皮细胞形态以及血液学/血清生化指标方面未观察到与治疗相关的异常。该肽可抑制性传播感染病原体的生长，但对HIV - 1和HIV - 2无效。
麦加宁 - A可作为一种有效且安全的阴道内避孕化合物，对性传播感染病原体具有额外的防护作用。
