Pant Neeraj Chandra, Singh Ravinder, Chauhan Aditi, Gupta Vijaya, Mavuduru Ravimohan S, Prabha Vijay, Sharma Prince
1Department of Microbiology, South Campus, Basic Medical Science (Block I), Panjab University, Sector 25, Chandigarh, 160014 India.
2Department of Urology, PGIMER, Chandigarh, India.
Indian J Microbiol. 2019 Mar;59(1):51-57. doi: 10.1007/s12088-018-0766-5. Epub 2018 Nov 8.
, isolated from the cervix of an adult female with unexplained infertility, was found to agglutinate human spermatozoa in vitro leading to their death. A genomic library of was generated using pSMART- vector-host system. Approximately 3500 transformants were screened and four showed sperm agglutinating activity. Sperm agglutinating proteins (SAPs) were partially purified from the positive transformants and were found to agglutinate sperms in vitro. Cloned ORFs in positive transformants were sequenced and ORF finder identified them as endonuclease, accessory secretory protein-Asp1, accessory secretory protein-Asp2 and signal transduction protein. Mannose was found to competitively inhibit sperm agglutination, indicating that SAPs in bind to mannose in glycoprotein receptors on the surface of sperms for agglutination. This is the first report on identification of SAPs which may be responsible for unexplained infertility in women and may be used as contraceptive agents.
从一名患有不明原因不孕症的成年女性子宫颈中分离出的[具体物质未提及]，在体外被发现能凝集人类精子并导致其死亡。使用pSMART - 载体 - 宿主系统构建了[具体物质未提及]的基因组文库。筛选了大约3500个转化体，其中四个显示出精子凝集活性。从阳性转化体中部分纯化了精子凝集蛋白（SAPs），并发现它们在体外能凝集精子。对阳性转化体中克隆的开放阅读框（ORFs）进行了测序，ORF查找器将它们鉴定为核酸内切酶、辅助分泌蛋白 - Asp1、辅助分泌蛋白 - Asp2和信号转导蛋白。发现甘露糖能竞争性抑制精子凝集，这表明[具体物质未提及]中的SAPs与精子表面糖蛋白受体中的甘露糖结合以进行凝集。这是关于鉴定可能导致女性不明原因不孕症且可能用作避孕剂的SAPs的首次报告。
