[小白菊内酯对大鼠血管平滑肌细胞增殖的影响及其机制研究]
[Sbudies on the effect of parthenolide on the proliferation of rat vascular smooth muscle cells and the mechanism].
作者信息
Weng Shao-xiang, Shan Jiang, Lin Xiao-xia, Fu Guo-sheng
机构信息
Department of Cardiology, Second Affiliated Hospital, Medical School, Zhejiang University, Hangzhou 310009, Zhejiang, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2003 Jul;28(7):647-50.
OBJECTIVE
To study the effect of parthenolide on the proliferation of vascular smooth muscle cell(VSMC) and its mechanism.
METHOD
Vascular smooth muscle cell was cultured, the protein levels of c-fos, c-myc, p15, p16, p18, p19 were measured by Western blot method, cell cycle were examined with flow cytometry, and the DNA synthesis was determined by [3H]-TdR incorporation.
RESULT
Parthenolide inhibited protein levels of c-fos, c-myc in a time-dependent manner but didn't affect the protein levels of p15, p16, p18, p19. Flow cytometric DNA analysis revealed that parthenolide increased significantly G0/G1 phase of VSMC and decreased S phase of VSMC in a dose-dependent manner. Parthenolide inhibited [3H]-TdR incorporation in a dose dependent manner.
CONCLUSION
Parthenolide may inhibit proliferation of VSMC by inhibiting the expressions of c-fos, c-myc, but not the expressions of p15, p16, p18, p19.
目的
研究小白菊内酯对血管平滑肌细胞（VSMC）增殖的影响及其机制。
方法
培养血管平滑肌细胞，采用蛋白质免疫印迹法检测c-fos、c-myc、p15、p16、p18、p19的蛋白水平，用流式细胞术检测细胞周期，通过[3H]-TdR掺入法测定DNA合成。
结果
小白菊内酯能时间依赖性地抑制c-fos、c-myc的蛋白水平，但不影响p15、p16、p18、p19的蛋白水平。流式细胞仪DNA分析显示，小白菊内酯能剂量依赖性地使VSMC的G0/G1期显著增加，S期减少。小白菊内酯能剂量依赖性地抑制[3H]-TdR掺入。
结论
小白菊内酯可能通过抑制c-fos、c-myc的表达，而不是p15、p16、p18、p19的表达来抑制VSMC的增殖。
Zotero 插件