Department of Pharmacology, School of Pharmacy, Jilin University, Changchun, China.
Basic Clin Pharmacol Toxicol. 2010 Aug;107(2):685-9. doi: 10.1111/j.1742-7843.2010.00560.x.
This study was designed to examine the effect of ginsenoside Rb3 on angiotensin (Ang) II-induced proliferation of cultured rat vascular smooth muscle cells (VSMCs). VSMCs proliferation was evaluated by [3H]Thymidine incorporation. The cell cycle was examined by flow cytometry. The expression of mRNA of proto-oncogene c-myc, c-fos and c-jun was observed by RT-PCR. Ginsenoside Rb3 had no effects on VSMCs proliferation in physiological condition. Ang II significantly increased the proliferation of VSMCs and the expression of mRNA of proto-oncogene c-myc, c-fos and c-jun. Ginsenoside Rb3 markedly inhibited Ang II-induced VSMCs proliferation. Concomitantly, ginsenoside Rb3 decreased cell cycle progression from G(0)/G(1) to S phase. Furthermore, ginsenoside Rb3 significantly attenuated the expression of mRNA of proto-oncogene c-myc, c-fos and c-jun. This study showed that ginsenoside Rb3 inhibited Ang II-induced VSMCs proliferation, at least in part by inhibiting Ang II-induced G(0)/G(1) to S phase transition and attenuating the expression of mRNA of c-fos, c-jun and c-myc. The findings may explain the beneficial effects of ginsenoside Rb3 in cardiovascular diseases, and it will be useful to develop prevention and therapeutics of cardiovascular diseases.
这项研究旨在探讨人参皂甙 Rb3 对血管平滑肌细胞（VSMCs）增殖的影响。通过[3H]胸腺嘧啶掺入法评估 VSMCs 的增殖。通过流式细胞术检查细胞周期。通过 RT-PCR 观察原癌基因 c-myc、c-fos 和 c-jun 的 mRNA 表达。在生理条件下，人参皂甙 Rb3 对 VSMCs 的增殖没有影响。血管紧张素 II（Ang II）显著增加了 VSMCs 的增殖和原癌基因 c-myc、c-fos 和 c-jun 的 mRNA 表达。人参皂甙 Rb3 明显抑制 Ang II 诱导的 VSMCs 增殖。同时，人参皂甙 Rb3 降低了细胞周期从 G0/G1 期向 S 期的进展。此外，人参皂甙 Rb3 显著减弱了原癌基因 c-myc、c-fos 和 c-jun 的 mRNA 表达。这项研究表明，人参皂甙 Rb3 通过抑制 Ang II 诱导的 G0/G1 期向 S 期的转变和减弱 c-fos、c-jun 和 c-myc 的 mRNA 表达来抑制 Ang II 诱导的 VSMCs 增殖。这一发现可能解释了人参皂甙 Rb3 在心血管疾病中的有益作用，并将有助于开发心血管疾病的预防和治疗方法。
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