Xu Yi-Zhou, Gao Yan, Li Pei-Zhang, Wang Ning-Fu, Xu Hai-Ying, Tong Guo-Xin
The First People's Hospital of Hangzhou, Hangzhou 310006, China.
Zhongguo Zhong Yao Za Zhi. 2006 Mar;31(6):490-3.
To observe the role of puerarin on the proliferation of vascular smooth muscle cells(VSMC) induced by thrombin (T) and the effect of puerarin on the c-fos and bcl-2 protein expression.
Cell number and cell cycle analysis using flow cytometry were adopted as two different indicators of effects on proliferation of VSMC. Western blot was used to indicate the changes of c-fos and bcl-2 protein after 24 h of treatment of T and puerarin.
1.5 x 10(-5) - 1.5 x 10(-3) mol x L(-1) puerarin could significantly suppress this stimulation of VSMC proliferation and DNA synthesis induced by T. Western blot demonstrated that after 24 hour of treatment with T and puerarin, T could significantly increase c-fos and bcl-2 protein and 1.5 x 10(-5) - 1.5 x 10(-3) mol x L(-1) puerain could significantly suppress this increase.
puerarin can suppress the proliferation and DNA synthesis of VSMC promoted by T. This inhibitory effects of puerarin are closely related with the suppression of c-fos and bcl-2 protein.
观察葛根素对凝血酶（T）诱导的血管平滑肌细胞（VSMC）增殖的作用以及葛根素对c-fos和bcl-2蛋白表达的影响。
采用细胞计数和流式细胞术分析细胞周期作为对VSMC增殖影响的两种不同指标。用蛋白质免疫印迹法检测T和葛根素处理24小时后c-fos和bcl-2蛋白的变化。
1.5×10⁻⁵ - 1.5×10⁻³mol·L⁻¹的葛根素能显著抑制T对VSMC增殖和DNA合成的刺激作用。蛋白质免疫印迹法显示，T和葛根素处理24小时后，T能显著增加c-fos和bcl-2蛋白表达，而1.5×10⁻⁵ - 1.5×10⁻³mol·L⁻¹的葛根素能显著抑制这种增加。
葛根素能抑制T促进的VSMC增殖和DNA合成。葛根素的这种抑制作用与抑制c-fos和bcl-2蛋白密切相关。
